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KMID : 0366919990110000375
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Sungkyun Pharmceutical Journal
1999 Volume.11 No. 0 p.375 ~ p.382
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Effect of Tauroursodeoxycholic Acid on Ischemia/Reperfusion Injury in Isolated Rat Heart
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Abstract
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Abstract - In this study, the effects of tauroursodeoxycholic acid (TUDCA) on ischemia/ reperfusion injury were investigated on isolated heart perfusion models. Hearts were perfused with oxygenated Krebs-Henseleit solution (pH 7.4, 37℃) on a Langendorff apparatus. After equilibration, isolated hearts were treated with TUDCA 100 and 200μM or vehicle (0.02% DMSO) for 10 min before the onset of ischemia in single treatment group. In 7 day pretreatment group, TUDCA 50, 100 and 200 mg/kg body weight were given orally for 7days before operation. After global ischemia (30 min), ischemic hearts were reperfused for 30 min. The physiological (i.e. heart rate, left ventricular developed pressure, coronary flow, double product, time to contracture formation) and biochemical (lactate dehydrogenase; LDH) parameters were evaluated. In vehicle-treated group, time to contracture formation was 810 sec during ischemia, LVDP was 34.0 mmHg at the endpoint of reperfusion and LDH activity in total reperfusion effluent was 34.3 U/L. Single treatment with TUDCA did not change the postischemic recovery of cardiac function, LDH and time to contracture compared with ischemic control group. TUDCA pretreatment showed the tendency to decrease LDH release and to increase time to contracture and coronary flow. Our findings suggest that TUDCA does not ameliorate ischemia/reperfusion-induced myocardial damage.
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KEYWORD
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